Coding

Part:BBa_K1059012:Design

Designed by: Wenjun Wang   Group: iGEM13_OUC-China   (2013-09-16)


RBS B0032+mamQ coding squence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 748
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because synthetic biology proposes standard prefix and sufix, so we add prefix and sufix by PCR before we ligease it onto plasmid PSB1C3.


Source

It comes from Magnetospirillum magneticum AMB-1 bacteria strain.We firstly design primers to get this gene squence by PCR from genome, and then use another primer to add RBS J23106, prefix and sufix onto it.

References